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Methods in Biotechnology

2. Methods in Biotechnology · Part-A: General Biotechnology

RESEARCH_METHODOLOGY Research Practice

How research is framed, designed, executed, interpreted and written up for publication.

17 sub-topics

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03

biostatistics (central tendency, probability and distributions - binomial, Poisson, Gaussian

  • STATISTICS
08

biochemical methods (ion-exchange, gel-filtration and affinity chromatography

  • METHODS.SEPARATION
12

UV/Vis spectrophotometry and Beer-Lambert law for protein and nucleic-acid quantification

  • METHODS.SPECTROSCOPY
13

fluorescence spectroscopy (excitation and emission, quenching, Stern-Volmer plots, FRET, fluorescence lifetime)

  • METHODS.SPECTROSCOPY
15

centrifugation (g value from RPM, density-gradient, sedimentation velocity and equilibrium, separation of subcellular components)

  • METHODS.SEPARATION
16

microscopy (bright field, phase contrast, fluorescence, confocal, electron)

  • METHODS.MICROSCOPY
17

fundamentals of X-ray, NMR and cryo-EM for biomolecular structure

  • METHODS.SPECTROSCOPY