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Recombinant DNA Technology

5. Recombinant DNA Technology · Part-A: General Biotechnology

RDNA Biotechnology

Cutting, joining, delivering and expressing DNA - the toolkit of molecular cloning.

16 sub-topics

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01

Enzymes used in rDNA technology

BIOMOL.ENZYMES

02

isolation and purification of genomic and plasmid DNA and RNA

RDNA

03

separation and characterization including Southern and Northern hybridization

PLANT.BREEDING

04

molecular cloning in bacterial and eukaryotic systems

RDNA

05

expression and purification of recombinant proteins using bacterial, animal and plant vectors

RDNA

06

western blotting

METHODS.IMMUNO

07

genomic and cDNA libraries

RDNA

08

plasmid, phage, cosmid, BAC and YAC vectors

RDNA

09

in-vitro mutagenesis and deletion techniques

GENETICS.MUTATION

10

gene knock-out

RDNA

11

isolation and amplification of specific nucleic acid sequences - PCR, RT-PCR, qRT-PCR

BIOMOL.STRUCTURE

12

DNA sequencing methods and genome-sequencing strategies

RDNA

13

analysis of gene expression at RNA and protein level

BIOMOL.STRUCTURE

14

microarray-based large-scale expression

GENOMICS_OMICS

15

DNA polymorphism analysis - RFLP, RAPD, AFLP

RDNA

16

biosafety regulations and IPR

IPR_ETHICS