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Recombinant DNA Technology

8. Recombinant DNA Technology · Section B (Graduate level)

RDNA Biotechnology

Cutting, joining, delivering and expressing DNA - the toolkit of molecular cloning.

16 sub-topics

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01

Restriction and modification enzymes

BIOMOL.ENZYMES

02

vectors - plasmid, bacteriophage and other viral vectors, cosmids, Ti plasmid, yeast artificial chromosome, mammalian and plant expression vectors

RDNA

03

cDNA and genomic DNA libraries

RDNA

04

gene isolation, cloning and expression

RDNA

05

transposons and gene targeting

CHROM.ORG

06

DNA labeling

RDNA

07

DNA sequencing

RDNA

08

polymerase chain reactions

RDNA

09

DNA fingerprinting

GENOMICS_OMICS

10

Southern and northern blotting

RDNA

11

in-situ hybridization

PLANT.BREEDING

12

RAPD

RDNA

13

RFLP

RDNA

14

site-directed mutagenesis

RDNA

15

gene transfer technologies

RDNA

16

gene therapy

MEDICAL_BIOTECH