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Recombinant DNA Technology and Other Tools

BT6: Recombinant DNA Technology and Other Tools · GATE BT

RDNA Biotechnology

Cutting, joining, delivering and expressing DNA - the toolkit of molecular cloning.

4 sub-topics

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01

rDNA technology (restriction and modification enzymes, vectors including plasmids, phage and viral vectors, cosmids, Ti plasmid, BAC and YAC, expression vectors, cDNA and genomic libraries, gene isolation and cloning, transposons and gene targeting)

  • RDNA
02

molecular tools (PCR, DNA and RNA labelling, Sanger and next-generation sequencing, Southern and northern blotting, in-situ hybridization, DNA fingerprinting, RAPD, RFLP, site-directed mutagenesis, CRISPR-Cas, biosensors, interaction tools, genomics and proteomics approaches)

  • RDNA
03

analytical tools (microscopy, spectroscopy, electrophoresis, microarrays, enzymatic assays, ELISA, RIA and immunohistochemistry, immunoblotting, flow cytometry, whole-genome and ChIP sequencing)

  • METHODS.SEPARATION
04

computational tools (bioinformatics resources, databases, sequence analysis and phylogeny, genomics, proteomics and metabolomics, gene prediction, functional annotation, structure prediction, metagenomics, metabolic engineering and systems biology)

  • GENOME_EDITING