Skip to content

A. Molecular Biology and Recombinant DNA methods

13. Methods in Biology · Paper II

METHODS.SEPARATION Techniques

Chromatography, electrophoresis and centrifugation - the three ways biomolecules are physically resolved.

18 sub-topics

Open any one for its reading list, most-cited papers and cross-exam appearances.

Sign in to mark sub-topics complete and watch this topic fill up.

01

Isolation and purification of RNA, DNA (genomic and plasmid) and proteins

RDNA

02

different separation methods

METHODS.SEPARATION

03

analysis of RNA, DNA and proteins by one and two dimensional gel electrophoresis

METHODS.SEPARATION

04

isoelectric focusing gels

METHODS.SEPARATION

05

molecular cloning of DNA or RNA fragments in bacterial and eukaryotic systems

RDNA

06

expression of recombinant proteins using bacterial, animal and plant vectors

RDNA

07

isolation of specific nucleic acid sequences

BIOMOL.STRUCTURE

08

generation of genomic and cDNA libraries in plasmid, phage, cosmid, BAC and YAC vectors

RDNA

09

in vitro mutagenesis and deletion techniques

GENETICS.MUTATION

10

gene knock out in bacterial and eukaryotic organisms

METHODS.SEPARATION

11

protein sequencing methods

BIOMOL.STRUCTURE

12

detection of post translational modification of proteins

MOLBIO.TRANSLATION

13

DNA sequencing methods

RDNA

14

strategies for genome sequencing

GENOMICS_OMICS

15

methods for analysis of gene expression at RNA and protein level

BIOMOL.STRUCTURE

16

large scale expression such as microarray based techniques

GENOMICS_OMICS

17

isolation, separation and analysis of carbohydrate and lipid molecules

BIOMOL.STRUCTURE

18

RFLP, RAPD and AFLP techniques

METHODS.SEPARATION