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Analytical Techniques

XL1.4 Analytical Techniques · XL1: Biochemistry

BIOPHYS.CHEM Biochemistry

The physical chemistry a biologist cannot skip: pH, buffers, reaction kinetics, thermodynamics and colligative properties.

15 sub-topics

Open any one for its reading list, most-cited papers and cross-exam appearances.

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01

Buffering against pH changes in biological systems

BIOPHYS.CHEM

02

the Henderson-Hasselbalch equation

BIOPHYS.CHEM

03

ion exchange, size exclusion and affinity chromatography

METHODS.SEPARATION

04

thin layer and paper chromatography

METHODS.SEPARATION

05

centrifugation

METHODS.SEPARATION

06

characterization of biomolecules by electrophoresis

METHODS.SEPARATION

07

analytical methods to study DNA-protein and protein-protein interactions

BIOMOL.STRUCTURE

08

Lambert-Beer law

BIOPHYS.CHEM

09

UV-visible and fluorescence spectroscopy

METHODS.SPECTROSCOPY

10

next-generation DNA sequencing

GENOMICS_OMICS

11

PCR

RDNA

12

site-directed mutagenesis

RDNA

13

antibody-antigen interaction

IMMUNOLOGY

14

immunological techniques - immunodiffusion, immuno-electrophoresis, RIA and ELISA, flow cytometry

METHODS.SEPARATION

15

monoclonal antibodies and their applications

IMMUNOLOGY